Table 5.
Assessment of fluorescent dye-albumin conjugates on FcRn binding
| Fluorophore Conjugate (ratio of dye to albumin) | pH 6 Kd, µM | pH 7.4 Kd, µM |
|---|---|---|
| CF-594 maleimide (1:3) | 1.5 | NB |
| Texas Red-X (1:1) | 5.4 | NB |
| Texas Red-X (4:1) | >40 | NB |
| Alexa 488 (4.5:1) | NB | NB |
| Fluorescein (6.4:1) | 69 | 1.0 |
| Alexa 568 (4:1) | 17 | NB |
| Texas Red-X (2:1) | 10 | NB |
| Texas Red-X (5:1) | >100 | NB |
| Texas Red (1:1) | 6 | 11 |
| Alexa 647 maleimide (1:1) | 11 | NB |
| DO BSA | NB | NB |
| No dye | 11.6 | >100 |
Kd, dissociation coefficient; NB, no binding; DQ BSA, dye quenched bovine serum albumin.