Fig 3. The 500 bp C-terminal of TE is involved in the stress response in vitro.
In order to evaluate the resistance of Δpks1-TE-C500, all strains were inoculated on PDA with a 5 μl suspension containing 1×106/ml conidia under specific conditions. For salt stress, 0.2, 0.4, and 0.6 M potassium chloride were added. For oxidative stress, 0.5, 1, 2, and 2.5 mM H2O2, 0.225, 0.45 and 0.9 mM SNAP, or 15, 30, and 60 μM vitamin K were added. For osmotic stress, 0.25, 0.5, and 1 M sorbitol were added. Then, the plates were incubated for 14 days at 25°C (The straight line represents the basic level on the fifth day (0.6cm)). For UV stress, the strains were irradiated with UV light for 5, 10, and 15 min. Compared to the wild-type, the growth of the Δpks1-TE-C500 decreased significantly with the increase of stress factor concentration or UV irradiation time, especially oxidative stress and UV irradiation. Statistical significance was determined by ANOVA test (ns, not significant; *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001).