Table 1. Strains and plasmids used in this study.
Strain or plasmid | Genotype or characteristic |
---|---|
SUMS0310 (CBS269.37) | Wild-type of F. monophora |
Δpks1-TE-C500 | Knock out 500 bp C-terminal of TE domain of F. monophora |
Com | Complemented strain ofΔpks1-TE-C500 |
pBHt2 | Hygromycin-resistant plasmid |
pBHt2-XhoI | pBHt2 containing XhoI |
pks1-TE-hph-pBHt2 | Plasmid containing the TE gene and hygromycin resistance gene |
pks1-TE-neo-pBHt2 | Plasmid containing the TE gene and geneticin resistance gene |
pks1-TE-C-neo-pBHt2 | Plasmid containing the TE gene complemented fragment and geneticin resistance gene |