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. 2022 Mar 28;3(1):65–80. doi: 10.12336/biomatertransl.2022.01.007

Table 1. Allograft bone cleaning processes.

Bone clean Methods/materials Source
Physical process Sonication U.S. Patent No. 5,797,871
Pressurised flow U.S. Patent No 5,513,662
High pressure washing with agitation and liquid stream U.S. Patent No. 5,333,625
Agitation/shaker the University of Miami Tissue Bank
Oscillating atmospheric pressure U.S. Patent No. 6,652,818
Chemical process Ethanol KR101272958B1
Polyoxyethylene-23 lauryl ether Allowash XG®
(n-Butyl) phosphate, betadyne, TritonX-100/TNBP BioCleanse®
0.5% to 5% chlorhexidine gluconate U.S. Patent No. 10,004,819
Sodium hypochlorite and hydrogen peroxide U.S. Patent No. 7,507,254
Combinational Sonication + detergent; water and alcohol U.S. Patent application 20080188939
Supercritical fluid U.S. Patent No. 5,725,579
Explosive decompression U.S. Patent No. 5,288,462