Figure 5. Intensity of osteogenic differentiation markers in cells cultured on a micropatterned substrate. (A–F) Relative fluorescent intensity of ALP, COL I, and OCN in MSCs (A, C, E) and MC3T3-E1 cells (B, D, F). Data are presented as mean ± SD (n = 3). *P < 0.05 (one-way analysis of variance followed by Tukey’s post hoc analysis). ALP: alkaline phosphatase; COL I: type I collagen; CON: control (freely spreading group); LL: large circles with large spacing; MSC: mesenchymal stem cell; OCN: osteocalcin; PTN: pattern; SL: small circles with large spacing; SS: small circles with small spacing.