Figure 6.

Liposome surface chemistry can be engineered to regulate the release rate of protein cargo. (A) Liposomal hydrogels feature three distinct release modalities based on cargo interaction with liposome surface chemistry. (B) Representative images and raw data for fluorescence recovery after photobleaching (FRAP) experiments. (C) FRAP data was used to calculate size-based diffusivity constants for model cargo (dextran macromolecules) within liposomal hydrogels. (D) In vitro release of his-tagged GFP from liposomal hydrogels engineered to engage the cargo through distinct mechanisms. Samples were maintained at 37°C throughout the study. (E) Area under the release time curve. (F) Release data were modeled using the Korsmayer-Peppas equation to calculate the transport constant. All data indicate mean and SEM of three independent replicate experiments. Statistical comparisons were made using a one-way ANOVA, and the false discovery rate (FDR) was controlled at 5% using the Benjamini, Krieger, and Yekutieli method.