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. 2022 Jun 21;13:900667. doi: 10.3389/fphar.2022.900667

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Copyright © 2022 Sui, Tveter, Bawagan, Buckendahl, Martinez, Jaffri, MacDonell, Wu, Duran, Shapses and Roopchand.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CBD alleviated bone loss in OVX mice. Areal bone mineral density (aBMD) and bone mineral content (BMC) of (A,B) whole body and individual bone samples of (C,D) femur, (E,F) tibia (G,H) humerus, and (I,J) spine as measured by DEXA. Data are presented as mean ± SD and were collected from 9 mice in OVX groups and 10 mice in SS groups, except for humerus data in the SS + CBD group where 1 humerus samples was not successfully excised. ROUT test was performed to detect outliers. 2-way ANOVA was performed followed by the Benjamini–Hochberg post-hoc test with FDR adjustment, q < 0.05.2-way ANOVA results are indicated under each graph. For posthoc test, different letters (a–c) indicate significant differences between groups.