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. Author manuscript; available in PMC: 2023 Apr 15.
Published in final edited form as: Gene. 2022 Jan 29;818:146199. doi: 10.1016/j.gene.2022.146199

Figure 3.

Figure 3.

Effect of FGFR1 and FGFR2 knockdown on the osteogenesis of hMSCs. A) siRNA knockdown efficiency by qPCR analysis from either three (FGFR1) and six (FGFR2) separate wells from a single experiment. B) siRNA knockdown efficiency as assessed by Western blot from a single experiment. C) ALP activity after siRNA transfection. hMSCs were cultured in osteogenic medium for 8 days. Absorbance at 405 nm was normalized against that of the scrambled siRNA-treated cells. Data were from six wells from a single experiment. D) Cell viability in maintenance media measured 4 days after siRNA transfection from triplicate wells from a single experiment. Absorbance data at 450 nm were normalized against that of the control scrambled siRNA-treated cells. E) Alizarin red staining in siRNA-treated cells for mineral deposition after 21 days in osteogenic media. Data from triplicate wells from a single experiment. F) Quantitative analysis of Alizarin red staining in (E).