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. 2001 Nov 15;29(22):4561–4569. doi: 10.1093/nar/29.22.4561

Figure 1.

Figure 1

A new isoform of eIF4E in S.pombe. (A) Northern blot results indicate the presence of two types of eIF4E-encoding mRNA in S.pombe. These were detected in separate hybridisations using DNA probes derived from the gene isolated previously (tif451; 23) and from the cDNA described in the present work (tif452), and the two lanes are compared here side-by-side. One strong major band is visible in each of the lanes; the tif451 mRNA is shorter than the tif452 mRNA. There is some cross-reaction with the tif452 mRNA evident in the tif451 lane. (B) Comparison of the protein sequences of S.pombe eIF4EI and eIF4E2, and of 4EHP (the human 4E homologous protein; 22). Identities are indicated by bold letters, similarities by asterisks. Asterisks above the sequences relate to the eIF4E1 and eIF4E2 sequences, whereas those below relate to eIF4E2 and 4EHP. The eight conserved tryptophan residues are numbered. (C) Gonnet series analysis using ClustalX (35). Schizosaccharomyces pombe eIF4E1 and eIF4E2 are more closely related to each other and to S.cerevisiae eIF4E than to the higher eukaryotic eIF4Es or to the various isoforms and 4E-related proteins in other organisms.