Fig. 6.

CD146 increasing radioresistance in GG16 cells. A, B GG16 control (pCMV-GFP) and CD146/GFP overexpressing cells were untreated or exposed to different doses of ionizing radiation (IR) and colony forming potential was monitored. Representative images of plates with colonies and survival curves are shown, **p < 0.01 by Student’s t test. C Western blots showing effect of 4 Gy IR in time on expression of CD146 and indicated DDR, and cell cycle regulatory proteins in GG16 GFP control and GG16-CD146/GFP cells cultured as differentiated cells. Endogenous CD146 expression is induced by radiation, and overexpression of CD146/GFP is associated with prolonged CHK1 induction, suppression of p53, and increased NF-kB signaling