FIG. 2.

Restriction enzyme patterns of cosmids pCPP704 (lanes 3, 7, and 11) and pCPP702 (lanes 4, 8, and 12) and a subclone of pCPP702, pCPP1051 (lanes 2, 6, and 10). Lane 1 has Eh318 genomic DNA; lanes 5, 9, and 13 have λ digested with HindIII as molecular weight markers. The DNA was digested to completion with EcoRI and XbaI (lanes 1 through 4), EcoRV (lanes 6 through 8), and EcoRI (lanes 10 through 12). The arrows indicate the fragments that hybridized with a 3.9-kb XbaI-HindIII fragment of pCPP1051 (see Fig. 3) that covered a region considered important for synthesis of pantocin A. The composite photograph was created with Adobe Photoshop 5.5.