FIG. 3.

Physical map of the 20.2-kb insert (top) from pCPP702 in pCPP1051. The 11.8-kb EcoRI fragment containing the Tn5 insertion 122 (middle) that was cloned into pBR325 to create pCPP726. This clone was used for marker exchange of insertion 122 into Eh318 to generate Eh421 (PanA^l). The positions of Tn5 insertion 122, which abolishes pantocin A production (filled lollipop), and insertion 326, which does not affect production (open lollipop), are shown at the bottom. The XbaI-HindIII fragment (solid gray bar, below map) was radioactively labeled for use as a probe in Southern blots. Abbreviations: A, ApaI; B, BamHI; C, ClaI; E, EcoRI; R, EcoRV; S, SaII; Sm, SmaI; X, XbaI. The figure was generated from Innovative Data Design MacDraft, converted to MacroMedia FreeHand 8, and printed from Adobe Photoshop 5.5.