TABLE 1.
Bacterial strains, phages, cosmids, and plasmids used in this study
| Strain, phage, cosmid or plasmid | Relevant characteristicsa | Reference or sourceb |
|---|---|---|
| Escherichia coli | ||
| JM109 | recA1 thiΔ (lac-proAB) lacZΔM15 | 39 |
| HB101 | SmrrecA13 | 39 |
| DH5β | F−recA1 lacZΔM15 thi-1 Nalr | 39 |
| LE392 | hsdR514 supE44 supF58 lacY1 galK2 galT22 metB1 trpR55 λ− | 39 |
| CC118 | Δ(ara-leu)7697 ΔlacX74 ΔphoA20 galE galK thi rpsE rpoB argE recA1 Spr | 32 |
| SM10(λpir) | thi-1 thr leu tonA lacY supE recA::RP4-2-Tc::Mu Kmrλpir | 34 |
| CGSC6151 | LamB204; resistant to lambda | M. Schwartz, E. coli Genetic Stock Center |
| Erwinia amylovora Ea273 | Infected apple, ATCC 49946 | |
| Pantoea agglomerans | ||
| Eh318 | Rpr | Rundle, CUCPB 2140, from apple leaves |
| Eh421 (PanA−) | Marker exchange mutant of Eh318, deficient in pantocin A; Kmr | This work, CUCPB 4189 |
| Eh439 (PanB−) | Marker exchange mutant of Eh318, deficient in pantocin B; Cmr | This work, CUCPB 4433 |
| Eh440 (PanAB−) | Marker exchange mutant of Eh318, deficient in pantocin A and B; Kmr Cmr | This work, CUCPB 4434 |
| Eh252 | Rundle, CUCPB 2050, from apple leaves | |
| Pantoea sp. Eh112Y | Billing, CUCPB 0119, from apple | |
| P. stewartii 2 | Woods (54), CUCPB 0176 | |
| Erwinia carotovora subsp. carotovora B12 | Received from A. K. Chatterjee, CUCPB 2260 | |
| Erwinia carotovora subsp. betavasculorum 101 | CUCPB 0306 | |
| Erwinia chrysanthemi 3665 | Received from A. Toussaint, CUCPB 2256 | |
| Enterobacter aerogenes 1422/77 | CUCPB 0294 | |
| Serratia marcescens Ser 101 | CUCPB 0155 | |
| Klebsiella pneumoniae | ATCC 15574, CUCPB 0309 |
|
| Pseudomonas putida W713 | S. E. Lindow, CUCPB 2002 | |
| Pseudomonas syringae pv. tomato DC3000 | D. Cuppels, CUCPB 4103 | |
| Xanthomonas campestris pv. pelargonii Xpel-1 | Geranium isolate | M. Daughtery, CUCPB 4672 |
| Agrobacterium tumefaciens C58 | CUCPB 0312 | |
| Phage λ467 | λb221 rex::Tn5 cI857 Oam29 Pam80 Kmr | 6 |
| Cosmids | ||
| pCPP702 | Spr Smr; contains genomic fragments cloned in pCPP9, responsible for synthesis of pantocin A | This work |
| pCPP704 | Spr Smr; contains genomic fragments cloned in pCPP9, responsible for synthesis of pantocin B | This work |
| Plasmids | ||
| pCPP9 | pGB2-mob λcos Spr | 2 |
| pRK2013 | helper plasmid; Kmr | 18 |
| pBluescript KS(+) | Apr; cloning vehicle | Stratagene Corp. |
| pCPP1051 | 8.4 and 11.8-kb EcoRI fragments of pCPP702 in pBluescript KS(+) | This work |
| pCPP719 | 32.7-kb BamHI fragment of pCPP704, religated on itself; Spr Smr | |
| pUT/mini-Tn5Cm | Apr Cmrmob (RP4) ori (R6K) | 13 |
| pCPP723 | 16-kb partial ClaI fragment of pCPP719 carrying mini-Tn5Cm insertion A14, cloned in pBR322; Apr Tcr Kmr | This work |
| pCPP726 | 17.6-kb EcoRI fragment of pCPP1051 carrying Tn5 insertion 122, cloned in pBR325; Apr Tcr Cmr Kmr | This work |
| pBR322 | Apr Tcr; cloning vehicle | 9 |
| pBR325 | Apr Tcr Cmr; cloning vehicle | 8 |
| pCPP745 | pCPP1051 with Tn5 insertion 122; Apr Spr Kmr | This work |
| pCPP810 | pCPP719 with mini-Tn5Cm insertion A14; Cmr Spr | This work, CUCPB 5070 |
a
Apr, Cmr, Kmr, Nalr, Rpr, Smr, Spr, Tcr, indicate resistance to ampicillin, chloramphenicol, kanamycin, nalidixic acid, rifampin, streptomycin, spectinomycin, and tetracycline, respectively.
b
ATCC, American Type Culture Collection; CUCPB; Cornell University Collection of Phytopathogenic Bacteria. Last name denotes isolator or distributor.