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. 2022 Jul 5;13:3875. doi: 10.1038/s41467-022-31479-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Scheme of the decomposition of HCM NPs in presence of Hyal. Fluorescence images (b) and corresponding fluorescence intensity (c) of HCM NPs (Ce6: 40 μg/mL) after incubation with Hyal at different concentrations for 12 h (n = 3 independent samples; mean ± SD). Release of Ce6 (d) and MNZ (e) from HCM NPs (Ce6: 40 μg/mL; MNZ: 20 μg/mL) under different conditions (n = 3 independent samples; mean ± SD). The confocal laser scanning microscopy (CLSM) images of MRSA biofilms (f) and SMCs (g) after incubation with HCM NPs (Ce6: 50 μg/mL; MNZ: 25 μg/mL) for different times. Scale bar is 200 μm. Hydrodynamic size (h) and TEM images (i) of HCM NPs after incubation with Hyal (100 unit/mL) for 24 h. j Relative absorbance of ABDA at 380 nm (OD₃₈₀) after incubation at various conditions under laser irradiation (635 nm, 20 mW/cm²) for different times. Three independent experiments were performed and representative results are shown in f, g, and i. Source data are provided as a Source Data file.