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. 2022 Jul 5;5:663. doi: 10.1038/s42003-022-03506-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Quantitative results of clone formation experiments. b Quantitative results of cell apoptosis experiments. c Cell migration was evaluated by wound healing assay and reduced by GPR87 knockdown. Quantification of relative wound closure. d Migration and invasion of A549 and H1299 cells transfected with GPR87 siRNA were evaluated by a modified Boyden chamber assay. Quantification of the migration and invasion cells. e Representative immunoblotting of EMT-related proteins. f Representative immunoblotting of PI3K signaling pathway-related proteins. g After the knockdown of GPR87, the LPA receptor (5 µM) was added to the culture medium and WB assayed PI3K signaling pathway activation.