Table 3.
Estimation of the copy number of transgenes in rice line G281 using resequencing data and ddPCR.
| Gene | PE-WGS | ddPCR assay | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| G281 | WT | Copy number | Quantified copies | RSD (%) | Copy number | |||||||||
| ADTgm | Dgm | Rgm | ADTwt | Dwt | Rwt | 1 | 2 | 3 | Mean | |||||
| HLF | 48.93 | 28.91 | 0.989 | 0 | 29.28 | 0.989 | 1.95 | 37160 | 37760 | 37150 | 37356.7 | 0.94 | 1.96 | |
| EPSPS | 53.76 | 28.91 | 0.989 | 0 | 29.28 | 0.989 | 2.14 | 35680 | 35800 | 35320 | 35600 | 0.70 | 1.87 | |
| Gt1 | 77.75 | 28.91 | 0.989 | 27.05 | 29.28 | 0.989 | 2.02 | / | / | / | / | / | / | |
| CYP81A6 RNAi | 50.86 | 28.91 | 0.989 | 0.59 | 29.28 | 0.989 | 2.00 | / | / | / | / | / | / | |
| Ubiquitin | 43.87 | 28.91 | 0.989 | 0.79 | 29.28 | 0.989 | 1.72 | / | / | / | / | / | / | |
| PEPC | 43.97 | 28.91 | 0.989 | 0 | 29.28 | 0.989 | 1.75 | / | / | / | / | / | / | |
| SPS | 25.11 | 28.91 | 0.989 | 25.14 | 29.28 | 0.989 | / | 18980 | 19000 | 19100 | 19026.7 | 0.34 | / | |
“Gt1”,rice glutelin promoter. “ CYP81A6 RNAi”, including a 3′ untranslated region (UTR), the RNAi sequence, and the CaMV35s promoter.“/” means did not detect.