Figure 5. BCAT1i/AKG causes nucleotide depletion in IDH^WT GBM cells.

(A) The heatmap of cellular metabolites in parental and BCAT1 KO#2 U251MG cells treated with vehicle or DMAKG (10 mM) for 24 hours (n = 5). (B and C) Analysis of the enriched metabolic pathways in parental and BCAT1 KO#2 U251MG cells treated with vehicle or DMAKG (10 mM) for 24 hours. (D) Mass spectrometry analysis of nucleotides in parental and BCAT1 KO#3 U251MG cells treated with vehicle, DMAKG (10 mM), KIC (1 mM), or both for 24 hours (mean ± SEM, n = 4).