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. 2022 Jul 1;82:104136. doi: 10.1016/j.ebiom.2022.104136

Figure 5.

Figure 5

γδT17 trafficking induced by GC treatment is dependent on CCR6. (A, B) Ear thickness of WT and Ccr6−/−(a) or CCR2−/− mice (b, n=5) from Day 1 to Day 21. Data are the representative of at least two independent experiments with similar results. Data represent as mean and error. ****P<0.0001 (two-way ANOVA). (c) Summarized neutrophil percentages. Neutrophils were gated on CD45+CD11b+ cells. Data are the representative of at least two independent experiments with similar results (n=3 in each group). *P<0.05 (one-way ANOVA). (d, e) Summarized IL-17 production by skin CD3+, dermal or LNs γδT, Vγ4, Vγ4Vδ4 T cells of WT, Ccr6−/− and Ccr2−/− mice from skin (d), cervical LNs (e, Left) or inguinal LNs (e, Right) on day 21 (n=6 in WT group, n=5 in Ccr6−/−/IMQ/Vas group, n=6 in Ccr6−/−/IMQ/GC group, n=3 in Ccr2−/− group). Data are representative of at least two independent experiments with similar results, ***P<0.001, **P<0.01, *P<0.05, (two-way ANOVA). (f) Ear thickness of WT and Ccr6−/− mice rechallenged with IMQ from Day 35 to Day 42. Data represent as mean and error. ****P<0.0001 (two-way ANOVA). (g) Representative HE stained skin sections from WT or CCR6 KO mice rechallenged with IMQ on day 42. Data in the bar figures are represented as means±SEM.