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. 2022 Jun 22;12:871135. doi: 10.3389/fcimb.2022.871135

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Copyright © 2022 Lu, Ning, Kang, Bai, Zhou, Kang, Wu, Tian, Zhao, Ma and Bai

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Antibody and complement detection of CnpB-immunized mice after M. tuberculosis intranasal infection. After M. tuberculosis H37Ra intranasal challenge for 8 weeks, antibodies and complements were detected. (A) Sera of CnpB immunized (CnpB) mice and unimmunized (UN) mice were collected for antibodies detection using ELISA (n=3). The collection time points was 8 weeks after infection. (B) The ratio of IgG2a/IgG1 in panel A was calculated (n=3). (C, D) The levels of complement C3/C5 in sera (n = 3) (C) and complement C3 in lung/spleen homogenate (n = 4) (D) were assayed by ELISA. (E) The levels of sIgA in BALF were assayed by ELISA. The results are shown as mean ± SD. *P < 0.05, **P < 0.01, ****P < 0.0001.