Fig. 2.

Proliferation, migration and angiogenesis of endothelial cells induced by nanohybrids and their different components. (A) The effects of QCS, 5QCS-1GO and 5QCS-1GO-PDA on endothelial cells proliferation and calcein-AM staining analysis. Scale bar: 200 μm. (B) The effects of 5QCS-1GO-PDA and QCS, 5QCS-1GO on the proliferation of endothelial cells were evaluated by EdU incorporation. Scale bar: 100 μm. (C–F) Cytoskeleton staining, scratch assay, transwell assay and tube formation were used to detect the effects of nano-hybrid compounds on migration and angiogenic ability of endothelial cells. Scale bar: 50 μm. (G–H) Cell cycle determination of the effect of nanohybrids on the cycle distribution of endothelial cells by flow cytometry. Data are represented as mean ± SD (n = 3, *p < 0.05, **p < 0.01, ***p < 0.001).