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. 2022 Jun 2;18:552–568. doi: 10.1016/j.bioactmat.2022.05.023

Fig. 7.

Fig. 7

Surface morphology, cell adhesion and osteogenic ability of the PLA/HA scaffolded by SLS. (A) Schematic diagram of PLA/HA scaffolds printed by SLS. (B)SEM images of the PLA/HA printed with different proportions with SLS. Scale bar: 500 μm. (C) Alkaline phosphatase and alizarin red staining of different proportions of the PLA/HA scaffolds. Scale bar: 100 μm. (D) qRT-PCR was used to detect the expression of osteogenic genes in MC3T3-E1 cells co-cultured with different proportions of PLA/HA scaffolds. (E–I) Western blotting of MC3T3-E1-associated osteogenic protein expression on PLA/HA scaffolds at different proportions. (J) MC3T3-E1 cells adhesion on the PLA/10%HA scaffold. Scale bar: 5 μm. (K–L) Confocal laser microscopic imaging of MC3T3-E1 cells cultured on the PLA/HA scaffolds staining at day 3 (green: live cells). Data are represented as mean ± SD (n = 3, *p < 0.05, **p < 0.01, ***p < 0.001).