Fig. 5.

Functional recovery of mice in different groups in vivo. (a) Schedule of spinal cord injury mice treated with RA@BSA@Cur NPs. IOCV: Injection of caudal vein, t.i.w: three times a week. (b) Fluorescence imaging of SCI mice post i.v. Injection of Cy5.5-labeled RA@BSA, Cur@BSA, RA@BSA@Cur NPs in vivo. (c&d) Fluorescence imaging and qualitative analysis of spinal cord, heart, liver, spleen, lung and kidney in SCI mice post i.v. Injection after 48 h. (e) Behavior recovery of regenerated spinal cord injury mice. (f) Lower limb motor function over time using BMS score. (g) Macrography of spinal cord tissues at 8 weeks post-surgery. (h) Microscopic observation and quantitative assessment of the β3-Tubulin⁺ neurons (yellow arrow) and GFAP⁺ astrocytes (red arrow) in the defects after 8 weeks post-implantation. (n = 3 independent samples). Statistical differences were determined by using the ANOVA with Bonferroni's multiple comparison test (*p < 0.05, **p < 0.01, ***p < 0.001, ns: no significant; a.u. means arbitrary unit).