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Knocked down of GUSB promoted the CD8+ T-cell exhaustion markers expression when co-cultured with antigen-specific CD8+ T cells isolated from 3 PBMC samples. HCC cells treated with sh-NC/sh-GUSB were co-cultured with antigen-specific CD8+ T cells, and the expression of common markers of CD8+ T-cell exhaustion (LAG3, PD1, and TIGIT) was measured by flow cytometry. *P < 0.05; **P < 0.01.
