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. 2022 Jun 22;13:876048. doi: 10.3389/fimmu.2022.876048

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Copyright © 2022 Kong, Zheng, Song, Zhang, Liu, Kang, Sun, Sun, Huang, Li, Rong, Wang, Tang and Xia

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Knocked down of GUSB inhibited the proliferation, invasion and migration of human HCC cells and up-regulated PD-L1 expression. (A) The growth curves of cells were plotted after transfection with sh-NC/sh-GUSB based on CCK-8 assay. (B) EdU assay was performed to assess cell proliferation of HCC cells transfected with sh-NC/sh-GUSB. Scar bar, 50 um. (C) Transwell invasion assay was performed to assess invasion of HCC cells transfected with sh-NC/sh-GUSB. Scar bar, 100 um. (D) Scratch assay was performed to assess the migration of HCC cells transfected with sh-NC/sh-GUSB. Scar bar, 250 um. (E, F) The protein expression of PD-L1 in HCC cells with GUSB knockdown. Panel (E) represents the variation of protein bands, and Panel (F) illustrates the protein gray value analysis. Each experiment was repeated three times. **P < 0.01; ***P < 0.001; ****P < 0.0001.