Figure 5.

Knockdown of GUSB enhanced the anti-HCC effect when combined with anti-PD1 therapy in vivo. (A) Schematic diagram of animal experiment. We injected Hepa1-6 cells (sh-NC, sh-GUSB, sh-NC + anti-PD1, and sh-GUSB + anti-PD1) into C57BL/6 mice. (B–D) The volume (B, C) and weight (D) statistics of subcutaneous tumors in the respective groups (sh-NC, sh-GUSB, sh-NC + anti-PD1, sh-GUSB + anti-PD1). (E) Immunohistochemistry result of CD8, Ki67, PD1, and PD-L1 expression in the respective groups. Scar bar, 50 um. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.