FIG. 5.

Alignment of the corrinoid domain of CmuA from H. chloromethanicum CM2^T with CmuA from M. chloromethanicum CM4^T, MtmC from M. barkeri, and MetH from E. coli. CmuA from H. chloromethanicum CM2^T was aligned with CmuA from M. chloromethanicum CM4^T (accession no. AJ011316), MtmC from M. barkeri (AF013713), and MetH from E. coli (P13009). Aligned sequences in GCG's MSF format were downloaded into the BoxShade 3.21 vs program, which highlighted similar residues (shaded boxes) and identical residues (black boxes). Asterisks indicate the D-x-H-x₂-G-x_41–42-SxL-x_24–28-GG motif, a motif consisting of conserved residues involved in cobalamin binding in methionine synthases and mutases (27).