Alignment of CmuB from H. chloromethanicum CM2T with CmuB from M. chloromethanicum CM4T, MtrH from M. barkeri, and MetH from E. coli. CmuB from H. chloromethanicum CM2T was aligned with CmuB from M. chloromethanicum CM4T (accession no. AJ011317), M. barkeri MtrH (AJ132817), and E. coli MetH (P13009). Aligned sequences in GCG's MSF format were downloaded into the BoxShade 3.21 vs program, which highlighted similar residues (shaded boxes) and identical residues (black boxes). Asterisks indicate a sequence pattern that is present in all eight MtrH-related sequences and in all nine MetH-related sequences, G-[EA]-x2-[TNG]-x47–57-[LIM]-x16–20-[DN]-[RSA]-x8-[GA]-x10–15-[NA]-S-x19–24-[AL]-x10–18-[GA]-x9–23-G-x6–8-D-x19–27-[KRA]-x8–10-[GA]-x-[HAS]-N (Swissprot release 38; EMBL release 55)(41).