TABLE 1.
Summary of H. chloromethanicum CM2T methyltransferase genes and adjacent open reading frames
| Gene or open reading frame | Length of polypeptide (amino acids) | Gene start end position (bp) | Inferred function | Sequence comparison for representative protein hit (% identity)a |
|---|---|---|---|---|
| folD | >85 | 203–<start C | Methylene tetrahydrofolate cyclohydrolase/ dehydrogenase | P. phosphoreum (40), A. fulgidus (39), E. coli FolD (22) |
| pduX | 185 | 1239–682 C | Propandiol dehydratase | S. enterica serovar Typhimurium PduX (39%) |
| orf153 | 153 | 2060–2521 | No match | |
| orf207 | 207 | 2521–3144 | Porin precursor | N. denitrificans (37%) |
| orf225 | 225 | 3275–3952 | No match | |
| cmuB | 311 | 4413–5348 | Methyltransferase | M. chloromethanicum CM4T CmuB (57) |
| cmuC | 370 | 5501–6613 | Methyltransferase | M. chloromethanicum CM4T CmuC (36) and orf414 (34) |
| cmuA | 612 | 6610–8448 | Methyltransferase and corrinoid protein | M. chloromethanicum CM4T CmuA (80) |
| fmdB | 110 | 8476–8808 | Transcriptional regulator | M. methylotrophus FmdB (23) |
| paaE | >240 | 8833–<9556 | Reductase | E. coli PaaE (33), P. putida TdnB (32) |
| end |
a
Levels of protein identity were determined by performing a National Center for Biotechnology Information BLAST database search analysis.