Table 1. The clinical performance of the RT-RAA using simplified RNA extraction or automatic RNA extraction compared with qRT-PCR as the reference method.
| Method | qRT-PCR | Performance characteristics | |||||
| Positive | Negative | Sensitivity (%) | Specificity (%) | Kappa | |||
| Abbreviations: RT-RAA=reverse transcription recombinase-aided amplification; qRT-RAA=reverse transcriptase real-time quantitative PCR. | |||||||
| RT-RAA (automatic RNA extraction) | Positive | 292 | 6 | 92.41 | 98.78 | 0.92 | |
| Negative | 24 | 486 | |||||
| Total (n=808) | 316 | 492 | |||||
| RT-RAA (simplified RNA extraction) | Positive | 12 | 0 | 40 | 100 | 0.39 | |
| Negative | 18 | 28 | |||||
| Total (n=58) | 30 | 28 | |||||