Fig. 5.

Effects of M2-EVs on multiple organ damage in vivo. (A) Representative H&E staining micrographs of lung, liver and spleen sections at 24 h after LPS challenge (scale bar = 20 μm for lungs and livers; scale bar = 100 μm for spleens). The enlarged figures show the detailed changes in lung and liver sections and the black arrows in splenic micrographs show the loss in boundary definitions. (B) Quantification of histological injury scores of the lung, liver and spleen by professional pathologists (n = 3). (C) Representative TUNEL staining micrographs of the lung, liver and spleen sections at 24 h after LPS challenge (scale bar = 100 μm). (D) Quantification of TUNEL⁺ cells in the lung, liver and spleen sections. (n = 3). (^⁎⁎⁎p<0.001, ^⁎⁎p < 0.01 vs CON group; ^###p<0.001, ^##p<0.01 vs LPS group).