Fig. 7.

M2-EVs inhibited cytokine production by mediating complex signaling networks. (A) An overview of miRNA contents within M2-EVs and the top 8 most abundant miRNAs are listed. (B) miRNA-gene network analysis and KEGG pathway enrichment analysis based on differentially expressed genes. (C) Measurement of cytokine TNF-α and IL-6 mRNA levels in LPS/IFN-γ-primed PMφ cotreated with M2-EVs and different miRNA inhibitors for 4 h. (D) Western blot analysis of the phosphorylation levels of p38MAPK, STAT3 and NF-κB in LPS/IFN-γ-primed PMφ cotreated with M2-EVs and miRNA inhibitors for 1 h. (E) Quantification of the phosphorylation levels of p38MAPK, STAT3 and NF-κB in different groups (n = 3; ^⁎⁎p < 0.01, ^⁎p<0.05 vs CON group; ^##p<0.01 vs LPS group). (F) Western blot analysis of HGF, IL-10, SOD2, TGFβ1 and TSG101 protein expression in M2 cells and EVs.