Table 2.

Imaging mass cytometry antibody panel

	Target	Clone	RRID	Metal	Incubation time	Temperature	Dilution
1	Pan-Keratin	C11 and AE1/AE3	N/A	106Pd	Overnight	4°C	50
2	Collagen I	EPR7785	N/A	115In	Overnight	4°C	50
3	HLA-DR	TAL 1B5	N/A	141Pr	5 h	RT	100
4	EGF-R	D38B1	N/A	142ND	Overnight	4°C	50
5	CD68	D4B9C	N/A	143ND	Overnight	4°C	100
6	CD11b	D6X1N	N/A	144ND	5 h	RT	100
7	CD4	EPR6855	AB_2864377	145ND	Indirect	4°C	50
8	CD8α	D8A8Y	N/A	146ND	5 h	RT	50
9	CD31	89C2	N/A	147Sm	Overnight	4°C	100
10	CD73	D7F9A	N/A	148ND	5 h	RT	100
11	CD69	EPR21814	AB_2891140	149Sm	Overnight	4°C	100
12	Granzyme B	D6E9W	N/A	150ND	5 h	RT	100
13	CD66b	G10F5	N/A	151Eu	5 h	RT	100
14	Ki-67	8D5	N/A	152Sm	Overnight	4°C	100
15	CD3	EP449E	N/A	153Eu	Overnight	4°C	50
16	TIM3	D5D5R(TM)	N/A	154Sm	5 h	RT	100
17	CD141	E7Y9P	N/A	155Gd	Overnight	4°C	50
18	NKG2A	LS-C165590	N/A	156Gd	5 h	RT	50
19	CD39	EPR20627	N/A	157Gd	5 h	RT	100
20	CD1c	EPR23189-196	AB_2884015	158Gd	5 h	RT	50
21	FOXp3	D608R	N/A	159Tb	Overnight	4°C	50
22	PD-1	D4W2J	N/A	160Gd	5 h	RT	50
23	DC-SIGN	NBP1-77284	N/A	161Dy	Overnight	4°C	50
24	IDO	D5J4E(TM)	N/A	162Dy	Overnight	4°C	100
25	CD14	D7A2T	N/A	163Dy	5 h	RT	100
26	CD204	J5HTR3	N/A	164Dy	5 h	RT	50
27	CD45RO	UCHL1	AB_2563752	165Ho	Overnight	4°C	100
28	D2-40	D2-40	N/A	166Er	Overnight	4°C	100
29	CD56	E7X9M	N/A	167Er	5 h	RT	100
30	CD103	EPR4166(2)	N/A	168Er	5 h	RT	50
31	CD38	EPR4106	AB_2864383	169Tm	Overnight	4°C	100
32	CD45RA	HI100	N/A	170Er	5 h	RT	100
33	CD15	BRA-4F1	N/A	171Yb	Overnight	4°C	100
34	Cleaved caspase-3	ASP175	N/A	172Yb	5 h	RT	100
35	CD163	EPR14643-36	N/A	173Yb	5 h	RT	50
36	CD7	EPR4242	AB_2889384	174Yb	5 h	RT	100
37	CD45	D9M8I	N/A	175Lu	5 h	RT	50
38	CD11c	EP1347Y	AB_2864379	176Yb	5 h	RT	100
39	Vimentin	D21H3	N/A	194Pt	Overnight	4°C	50
40	HLA-G	MEM-G2	N/A	198Pt	5 h	RT	100
41	αSMA	D4K9N	N/A	209Bi	5 h	RT	100
42	Βcatenin	D10A8	N/A	89Y	Overnight	4°C	100

In bold are the markers that are overlapping with the SMC panel.