Antibodies |
|
CyTOF antibodies in Table 2. |
In Table 2 of this paper |
In Table 2 of this paper |
Collagen IV (Dako, M0785), |
Dako |
M0785 |
Fibronectin (Sigma-Aldrich, F3648) |
Sigma-Aldrich |
F3648 |
Laminin (Sigma-Aldrich, L9393). |
Sigma-Aldrich |
L9393 |
goat-anti-mouse or rabbit Ig-HRP (Dako, K500711) |
Dako |
K500711 |
goat anti-rabbit IgG-AF546 (Invitrogen, A-11035) |
Invitrogen |
A-11035 |
goat anti-mouse IgG1-AF488 (Invitrogen, A-21121) |
Invitrogen |
A-21121 |
|
Chemicals, and peptides |
|
H2O2 (Merck, 107209). |
Merck |
107209 |
3,3′-Diaminobenzidine (Dako, K5007) |
Dako |
K5007 |
xylene (VWR, 28975.291) |
VWR |
28975.291 |
ethanol (Merck, 1009831000) |
Merck |
1009831000 |
citrate buffer (BioLegend, 420902) |
BioLegend |
420902 |
superblock (ThermoScientific, 37580) |
ThermoScientific |
37580 |
Hematoxylin Sigma-Aldrich, 51275 |
Sigma-Aldrich |
51275 |
DAPI-ProLong Gold (Invitrogen, P36941) |
Invitrogen |
P36941 |
mounting medium (VWR, 3801731) |
VWR |
3801731 |
Iridium (125 μM) |
Fluidigm |
201192A |
|
Deposited data |
|
Raw MCD files |
This paper |
https://data.mendeley.com/datasets/gs2bj33r6f/draft?a=4e897e07-74d4-493a-b145-9cf00e2c2a33 |
|
Software and algorithms |
|
Ilastik (v1.3.3) |
|
https://www.ilastik.org/ |
Cellprofiler (v2.3.1), |
|
https://cellprofiler.org/ |
ImaCytE |
|
https://github.com/biovault/ImaCytE |
Graphpad Prism (V8) |
|
https://www.graphpad.com/scientific-software/prism/ |
MCD viewer |
|
https://www.fluidigm.com/products-services/software |
|
Other |
|
glass slides (Superfrost plus) |
VWR |
631–9483 |