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. 2022 Jun 20;25(7):104648. doi: 10.1016/j.isci.2022.104648
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

CyTOF antibodies in Table 2. In Table 2 of this paper In Table 2 of this paper
Collagen IV (Dako, M0785), Dako M0785
Fibronectin (Sigma-Aldrich, F3648) Sigma-Aldrich F3648
Laminin (Sigma-Aldrich, L9393). Sigma-Aldrich L9393
goat-anti-mouse or rabbit Ig-HRP (Dako, K500711) Dako K500711
goat anti-rabbit IgG-AF546 (Invitrogen, A-11035) Invitrogen A-11035
goat anti-mouse IgG1-AF488 (Invitrogen, A-21121) Invitrogen A-21121

Chemicals, and peptides

H2O2 (Merck, 107209). Merck 107209
3,3′-Diaminobenzidine (Dako, K5007) Dako K5007
xylene (VWR, 28975.291) VWR 28975.291
ethanol (Merck, 1009831000) Merck 1009831000
citrate buffer (BioLegend, 420902) BioLegend 420902
superblock (ThermoScientific, 37580) ThermoScientific 37580
Hematoxylin Sigma-Aldrich, 51275 Sigma-Aldrich 51275
DAPI-ProLong Gold (Invitrogen, P36941) Invitrogen P36941
mounting medium (VWR, 3801731) VWR 3801731
Iridium (125 μM) Fluidigm 201192A

Deposited data

Raw MCD files This paper https://data.mendeley.com/datasets/gs2bj33r6f/draft?a=4e897e07-74d4-493a-b145-9cf00e2c2a33

Software and algorithms

Ilastik (v1.3.3) https://www.ilastik.org/
Cellprofiler (v2.3.1), https://cellprofiler.org/
ImaCytE https://github.com/biovault/ImaCytE
Graphpad Prism (V8) https://www.graphpad.com/scientific-software/prism/
MCD viewer https://www.fluidigm.com/products-services/software

Other

glass slides (Superfrost plus) VWR 631–9483