. 2022 Jun 20;42(6):929–936. [Article in Chinese] doi: 10.12122/j.issn.1673-4254.2022.06.18

1.

RT-PCR基因引物序列

Primer sequences for RT-PCR

Gene	Primer sequences
AFP	Forward: 5'-AAGTTTAGCTGACCTGGCTAC-3'
AFP	Reverse: 5'-AAAGTGGGATCGATGCTGGAG-3'
FOXA2	Forward: 5'-AAATCTCAGCCTCCCAACCG-3'
FOXA2	Reverse: 5'-CACCGCTCCCAGCATACTTT -3'
GATA-6	Forward: 5'-TCAAAGACTTGCTCTGGTAATAGC-3'
GATA-6	Reverse: 5'-GTTCTCGGGATTGGTGCTCT-3'
ACTA	Forward: 5'-TCCGGGACATCAAGGAGAAACT-3'
ACTA	Reverse: 5'-CCCATCAGGCAACTCGTAACTC-3'
LPL	Forward: 5'-CCTCCTCCTCAAGGGAAAGC-3'
LPL	Reverse: 5'-AATCTCTTCTTTGGTCGGCG-3'
SPP	Forward: 5'-ACGCCGACCAAGGAAAACTC-3'
SPP	Reverse: 5'-TGCTTCTGAGATGGGTCAGG-3'
PAX6	Forward: 5'-CCATCACCAATCAGCATAGGA-3'
PAX6	Reverse: 5'-GCTCGAATATGGGGCTCTGA-3'
VIMENTIN	Forward: 5'-GGATGCCCTTAAAGGAACCAATGA-3'
VIMENTIN	Reverse: 5'-GGAGGAAAAGTTTGGAAGAGGC-3'
NESTIN	Forward: 5'-GCCTACAGAGCCAGATCGC-3'
NESTIN	Reverse: 5'-CTCCAGCTTGGGGTCCTGAAA-3'
HIF-1α	Forward: 5'-CTGCAACATGGAAGGTATTGC-3'
HIF-1α	Reverse: 5'-GGCTCATATCCCATCAATTCGGT-3'
β-catenin	Forward: 5'-GAGGAGCAGCTTCAGTCCC-3'
β-catenin	Reverse: 5'-TCCAACTCCATCAAATCAGCTTG-3'
VEGFA	Forward: 5'-ACAACAAATGTGAATGCAGACCA-3'
VEGFA	Forward: 5'-TCACATCTGCAAGTACGTTCG-3'
β-actin	Forward: 5'-GCCGCCAGCTCACCAT-3'
β-actin	Reverse: 5'-TCGTCGCCCACATAGGAATC-3'