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. 2022 Jun 9;298(7):102116. doi: 10.1016/j.jbc.2022.102116

Figure 1.

Figure 1

Construction of an ovariectomized (OVX) mouse model.A, schematic representation of the construction of an OVX mouse model for further investigation (n = 6). The experiments performed on the construction of OVX mouse model were included and some of the images such as C, E, and F were shown in the schematic representation. B, body weights of the sham and OVX groups at 2, 4, 6, and 8 weeks. C, the right femurs of all the mice were collected and subjected to micro-CT scanning and images were captured. The scale bars represent 500 μm. D, the bone mineral density (BMD), bone volume to tissue volume (BV/TV, %), trabecular thickness (Tb. Th, mm), and trabecular number (Tb. N, 1/mm) of each sample were measured. E and F, histological analysis with H&E and immunohistochemical staining with TRAP were conducted using thigh bone tissue slices of sham and OVX mice. OB.N/BS(/mm), OB.S/BS (%), OC.N/BS(/mm), and OC.S/BS (%) were measured with ImageJ software. The scale bars for main panels represent 200 μm. The scale bars for the zoom magnification panels represent 50 μm. G and H, the expression levels of the osteoclast-related genes TRAP, CTSK, and NFATc1 and, the osteoblast-related genes RUNX2, ALP, and OPN, in bone tissues from each group were measured by qRT-PCR. β-Actin was used as an internal reference gene. All the data are expressed as the means ± SDs. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. micro-CT, microcomputed tomography; qRT-PCR; quantitative RT-PCR.