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. 2022 Jul 6;5:669. doi: 10.1038/s42003-022-03630-3

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Nanobody-based western blotting assays of SARS-CoV-2 spikes: lysates of HEK293T cells expressing GFP or C-terminally C9-tagged SARS-CoV-2 spike (D614G) variants (indicated with numbers) were blotted with the indicated nanobodies. b–d Sandwich ELISA: b signals of gradually diluted sample concentrations using P158 as the capture antibody and P86 or P543 (b, c) or P543-Fc (d) as the detection antibodies are graphed as a mean of the measurements from two technical replicates. In c, sequentially diluted lysates of HEK293T cells expressing the original SARS-CoV-2 spike (D614G) or Beta variant (D614G, N501Y, E484K and K417N) were assayed. Data are representative of one independent experiment out of two biologically independent experiments. e A photograph of the results from lateral flow nitrocellulose membrane assays: P158 for the capture line (allow head) and P86 for the detection beads; 300 ng of the purified SARS-CoV-2 spike Delta variant (D614G, L452R and T478K) (+) or PBS (–) was spotted.