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. 2022 Jul 6;5:669. doi: 10.1038/s42003-022-03630-3

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Nanobody-based western blotting assays of SARS-CoV-2 spikes. HEK293T cell lysates (C: as control of mock transfection) expressing the C-terminally C9-tagged SARS-CoV-2 spike proteins (W: Wuhan-1) and one of the Omicron variant (O: Omicron BA.1) were blotted with an anti-C9 antibody and the indicated nanobodies. b Flow cytometric analyses of DT40 cells. Log scaled signals (x-axis) of the indicated eight nanobodies with parent cells (black) and cells stably expressing the SARS-CoV-2 Omicron spike protein (red) were counted (y-axis) and plotted.