Figure 2.

Regulatory effect of PB2 on redoxosomes signaling in Mes13 cells. (A) Cells were treated with PB2 (10 and 25 µM) under HG conditions (25 mM) for 12 h. The expression levels of redoxosome-related proteins were assessed by western blot analysis. (B) Cells were treated with RAC1 inhibitor (N, 80 µM) or NOX inhibitor (V, 10 µM) under HG conditions (25 mM) for 12 h at 37˚C. ROS generation was assessed using a ROS assay kit and H₂O₂ generation was assessed using the H₂O₂ detection kit, and results were statistically analyzed. All data are presented as the mean ± SD of three independent experiments. ^**P<0.01 vs. control; ^##P<0.01 vs. HG. PB2, procyanidin B2; HG, high glucose; N, NSC23766; V, VAS2870; ROS, reactive oxygen species; NOX, NADPH oxidase.