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. 2022 Jun 7;24(2):496. doi: 10.3892/etm.2022.11423

Figure 3.

Figure 3

Protective effect of PB2 against HG-induced extracellular matrix accumulation in Mes13 cells. (A) Cells were treated with PB2 (10 and 25 µM) under HG conditions (25 mM) for 12 h. The expression levels of collagen IV and fibronectin were assessed by western blot analysis and statistically analyzed. (B) Cells were treated with PB2 (10 and 25 µM) under HG conditions (25 mM) for 12 h. The expression levels of TGF-β1/SMAD signaling-related proteins were assessed by western blot analysis and statistically analyzed. (C) Cells were treated with TGF-β1/SMAD inhibitor (LY2109761, 10 µM) under HG conditions (25 mM) for 12 h at 37˚C. The expression levels of collagen IV and fibronectin were assessed by western blot analysis and statistically analyzed. All data are presented as the mean ± SD of three independent experiments. **P<0.01 vs. control; ##P<0.01 vs. HG. PB2, procyanidin B2; HG, high glucose; p-, phosphorylated.