Figure 4.

Protective effect of PB2 against HG-induced inflammation in Mes13 cells. Cells were treated with PB2 (10 and 25 µM) under HG conditions (25 mM) for 12 h. (A) Levels of IL-1β and TNF-α was assessed by enzyme-linked immunosorbent assay and statistically analyzed. (B) Localization of p65 (NF-κB) was assessed by immunofluorescence. Scale bar, 25 µm. All data are presented as the mean ± SD of three independent experiments. ^**P<0.01 vs. control; ^##P<0.01 vs. HG. PB2, procyanidin B2; HG, high glucose.