Figure 3.

Schematic representation of mRNA-LNP morphologies. At the far left, a spherical particle with mRNA and the lipid fully associated is depicted. The formation of a bleb involves the creation of a protrusion on the LNP characterized by a darkened, thicker periphery consistent with enrichment of the DSPC as the most mass dense component present. This aqueous compartment can occur to varying degrees and may or may not be occupied by mRNA (blue depicts aqueous; mottled surface depicts mRNA). In the extreme of dissociation, at right, mRNA-loaded or unloaded liposomes may also be formed as minor components.