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. 2022 Jun 17;19(7):2022–2031. doi: 10.1021/acs.molpharmaceut.2c00092

Figure 4.

Figure 4

Differential scanning calorimetry thermograms: (A) an unencapsulated mRNA; (B) same mRNA encapsulated in LNP, both in 20 mM Tris, 8% sucrose, and pH 7.4 buffer; (C) bovine serum albumin in 5 mM sodium citrate, pH 6.5 buffer; (D) mRNA-LNP sample from (B) reheated (after it had been heated to 95 °C and then cooled back to room temperature). In each panel, the experimental data is shown as the black line together with the mathematical fit in red. Gaussian components of the fit are shown as a color gradient together with the midpoint temperatures of each transition. Data were recorded using a MicroCal PEAQ-DSC capillary differential scanning microcalorimeter (Malvern-Panalytical, Malvern, United Kingdom) with a scanning rate of 1.5 °C/min.