FIGURE 3.
(A) SDS-PAGE analysis of the high-salt precipitation. An Invitrogen™ Mark12™ Unstained Standard (lane S) was used and the target molecule is indicated by arrows. Molecular weights of the selected proteins contained in the standard are shown on the left. The lanes are starting material (lane 1); supernatant (lane 2) and pellet (lane 3) after the high-salt precipitation; and supernatant (lane 4) and pellet (lane 5) after the centrifugation of the dissolved precipitation pellet. (B) Purity and target molecule yield per ml starting material were evaluated using image analysis of the SDS-PAGE gel.
