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. 2022 Jun 22;10:878838. doi: 10.3389/fbioe.2022.878838

TABLE 3.

Formulated ELP[V2Y-45] solutions of the three downstream processes were analyzed with regard to the target molecule’s molecular weight, its purity which was calculated from its band intensities in SDS-PAGE, the final target molecule yield, formulation buffer conductivity, and nucleic acid contamination in the final formulation (n = 3).

Process Molecular weight Purity Yield Conductivity A260/A280
(Da) (%) (mg ELP[V2Y-45]/ml starting material) (mS/cm)
HSP 21,594.2 ± 0.8 60.5 ± 3.9 3.72 ± 0.01 4.70 ± 0.04 0.99 ± 0.01
ITC 21,589.7 ± 1.1 88.0 ± 4.4 2.31 ± 0.09 3.62 ± 0.01 0.52 ± 0.01
IMAC 21,588.4 ± 0.1 86.5 ± 0.9 1.92 ± 0.08 3.59 ± 0.01 0.46 ± 0.00