TABLE 3.
Formulated ELP[V2Y-45] solutions of the three downstream processes were analyzed with regard to the target molecule’s molecular weight, its purity which was calculated from its band intensities in SDS-PAGE, the final target molecule yield, formulation buffer conductivity, and nucleic acid contamination in the final formulation (n = 3).
| Process | Molecular weight | Purity | Yield | Conductivity | A260/A280 |
|---|---|---|---|---|---|
| (Da) | (%) | (mg ELP[V2Y-45]/ml starting material) | (mS/cm) | ||
| HSP | 21,594.2 ± 0.8 | 60.5 ± 3.9 | 3.72 ± 0.01 | 4.70 ± 0.04 | 0.99 ± 0.01 |
| ITC | 21,589.7 ± 1.1 | 88.0 ± 4.4 | 2.31 ± 0.09 | 3.62 ± 0.01 | 0.52 ± 0.01 |
| IMAC | 21,588.4 ± 0.1 | 86.5 ± 0.9 | 1.92 ± 0.08 | 3.59 ± 0.01 | 0.46 ± 0.00 |