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| Initial nuclei number/tube | Average RNA concentration 1 (ng/μL) | Total amount of RNA (μg/tube) | A 260/280 |
| 50000 | 8898.5 | 133.5 | 2.19 |
| 25000 | 8484.2 | 127.3 | 2.15 |
| 10000 | 7508.4 | 112.6 | 2.11 |
| 5000 | 3215.5 | 48.2 | 2.03 |
| 2500 | 1012.7 | 15.2 | 1.98 |
| 1000 | 139.0 | 2.1 | 1.97 |
