Table 8. Recipe of NST buffer .

( N onidet P40 with S alts and T ris buffer)

Reagents	Initial Concentration	Final Concentration	Dilution Fold	Final Volume (10 mL)
NaCl	5,000 mM	146 mM	34.25	292 μL
Tris-HCl pH 7.5	1,000 mM	5 mM	200	50 μL
Tris-HCl pH 8	1,000 mM	5 mM	200	50 μL
CaCl 2	1,000 mM	1 mM	1000	10 μL
MgCl 2	1,000 mM	21 mM	47.62	210 μL
BSA	10%	0.05%	200	50 μL
IGEPAL CA-630	10%	0.20%	50	200 μL
Nuclease-free water	/	/	/	9138 μL