Table 9. Recipe of 2× Dialysis buffer .

Reagents	Initial Concentration	Final Concentration	Dilution Fold	Final Volume (10 mL)
HEPES(K+) pH 7.2	500 mM	100 mM	5	2 mL
NaCl	5,000 mM	200 mM	25	400 μL
EDTA	500 mM	0.2 mM	2500	4 μL
DTT	500 mM	2 mM	250	40 μL
Triton X-100	10%	0.20%	50	200 μL
Glycerol	100%	20%	5	2 mL
Nuclease-free water	/	/	/	5,356 μL