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| Reagents | Initial Concentration | Final Concentration | Dilution Fold | Final Volume (50 μL) |
| Tn5MErev/Tn5ME-A | 50 μM | 4 μM | 12.5 | 4 μL |
| Glycerol | 100% | 40% | 2.5 | 20 μL |
| 2× Dialysis buffer | / | / | / | 15.58 μL |
| pA-Tn5 | 55.55 μM | 2 μM | 27.76 | 1.81 μL |
| Nuclease-free water | / | / | / | 8.61 μL |
