Table 15. Recipe of FACT-seq Dig-washing buffer (for 8 libraries) .

Reagents	Initial Concentration	Final Concentration	Dilution Fold	Final Volume (15 mL)
HEPES(K+) pH 7.6	500 mM	20 mM	25	600 μL
NaCl	5,000 mM	150 mM	33.33	450 μL
Spermidine	2,000 mM	0.5 mM	4,000	3.75 μL
Digitonin	5%	0.05%	100	150 μL
IGEPAL CA-630	10%	0.01%	1,000	15 μL
BSA	10%	1%	10	1,500 μL
Nuclease-free water (with protease inhibitor added)		/	/	12.28 mL