Table 16. Recipe of FACT-seq Dig-300 buffer (for 8 libraries) .

Reagents	Initial Concentration	Final Concentration	Dilution Fold	Final Volume (2 mL)
HEPES (K+) pH 7.6	500 mM	20 mM	25	80 μL
NaCl	5,000 mM	300 mM	16.67	120 μL
Spermidine	2,000 mM	0.5 mM	4,000	0.5 μL
Digitonin	5%	0.05%	100	20 μL
IGEPAL CA-630	10%	0.01%	1000	2 μL
MgCl 2	1,000 mM	10 mM	100	20 μL
Nuclease-free water (with protease inhibitor added)		/	/	1,757.5 μL